FM drop out mixture, minus Arginine and w/o Lysine, 6700 mg/l

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FM drop out mixture, minus Arginine and w/o Lysine, 6700 mg/lThe FM drop out mixture, minus Arginine and without Lysine, is based on the Amino acids present in FM Minimal medium. These Amino acids form a basis for many Drop Out mixtures to select for auxotrophic requirements and transformants. Each FM Drop Out mixture contain all components except for one or two essential Amino acids, i. e. the dropped out supplements. FM Amino acids drop out mixtures are used in combination with FM Minimal medium w o Amino

01 Suspend 35

2 K2HPO4 9

5x is a 5x concentrate and has to be diluted to a 1x concentration and subsequently supplemented with an appropriate carbon source

Cold Spring Harbour laboratory

clinical microbiology

4 Riboflavin 0

1% Store at 2 - 8°C in dark

2 at 25°C Suspend 38 gram powdered medium in 1 litre distilled water

Yeast extract 5 Dextrose 30 Total 35

Improved media for growing plasmids and cosmid clones

This formulation supports the growth of most strains that are capable of growing on HL5

Addition of magnesium and calcium increases the growth of the recombinant cells